Production and Characterization of Exopolysaccharide Produced by Alcaligenes Faecalis B14 Isolated from Indigenous Soil
نویسندگان
چکیده
Exo-polysaccharides (EPS) are environment friendly natural polymers secreted by microorganisms in the surrounding medium. Due to the presence of unique structural composition, EPS shows diverse applications such as in food formulations, pharmaceutical, and cement based construction industry, etc. In the present investigation, the bacteria producing higher exopolysaccharide was screened among bacteria isolated from indigenous soil samples. The characterization of bacteria was done by biochemical and 16 S rDNA analysis. The investigation was done to determine the optimal variables of nutritional and environmental conditions to get maximum EPS production from the isolated bacteria. Glucose, yeast extract and MgSO4 enhanced the EPS yield at 30°C incubation temperature. Structural elucidation of the exo-polysaccharide was done using different spectroscopic techniques like H NMR, FTIR, and GC-MS. The monomeric composition of EPS contains glucose, rhamnose, glucuronic acid and mannose similar to welan gum. Exo-polysaccharide showed good emulsification property and viscosity that indicated its possible use as an additive in food industry.
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